Where academic tradition
meets the exciting future

You are here: TUCS > PUBLICATIONS > Publication Search > Data Combination from Multiple...

Data Combination from Multiple Matrix-Assisted Laser Desorption/Ionization (MALDI) Matrices: Opportunities and Limitations for MALDI Analysis.

Petri Kouvonen, Eeva M. Rainio, Veronika Suni, Päivi Koskinen, Garry L. Corthals, Data Combination from Multiple Matrix-Assisted Laser Desorption/Ionization (MALDI) Matrices: Opportunities and Limitations for MALDI Analysis.. Rapid communications in mass spectrometry 24(23), 3493–5, 2010.

Abstract:

Protein phosphorylation determination and dynamic changes in phosphorylation are amongst the most studied and pursued biological events today in life sciences. The analysis of phosphorylation now almost exclusively lies with MS-based methods, as these are more efficient, specific and reliable than other analytical approaches. Further developments of MS methods in phosphoproteomics are still necessary to discover and validate more phosphorylation sites, allow the measurement of the minute quantities of phosphoproteins in cells and tissues, and the stoichiometry of phosphorylation, which is typically low and often transient. The work described here reports on the use of multiple MALDI matrices to improve combined MS/MS spectral quality, and
subsequent validation of phosphorylation sites. It also offers an insight into the virtues of bypassing enrichment methods when low levels of sample exist, by comparing multimatrix-MALDI-MS/MS performance to an integrated TiO2 enrichment, nanoLC/ESI-MS/MS workflow for comprehensive single protein analysis.

BibTeX entry:

@ARTICLE{jKoRaSuKoCo10a,
  title = {Data Combination from Multiple Matrix-Assisted Laser Desorption/Ionization (MALDI) Matrices: Opportunities and Limitations for MALDI Analysis.},
  author = {Kouvonen, Petri and Rainio, Eeva M. and Suni, Veronika and Koskinen, Päivi and Corthals, Garry L.},
  journal = {Rapid communications in mass spectrometry},
  volume = {24},
  number = {23},
  pages = {3493–5},
  year = {2010},
  keywords = {phosphorylation, MALDI, phosphoproteins, MALDI-MS/MS, Titanium Dioxide, TiO2},
}

Belongs to TUCS Research Unit(s): Other

Publication Forum rating of this publication: level 2

Edit publication