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Biochemical and Clinical Approaches in Evaluating the Prognosis of Colonic Cancer

Kati Talvinen, Johannes Tuikkala, Juha Grönroos, Heikki Huhtinen, Pauliina Kronqvist, Tero Aittokallio, Olli S. Nevalainen, Heikki Hiekkanen, Timo Nevalainen, Jari Sundström, Biochemical and Clinical Approaches in Evaluating the Prognosis of Colonic Cancer. Anticancer Research 26(6), 2006.

Abstract:

Background: Colorectal adenocarcinoma is a
common malignant neoplasm in the western world. To achieve
optimal treatment results, the estimation of risk of recurrence
should be as accurate as possible. Materials and Methods:
Tissue material from tumour and normal mucosa were taken
from six patients and were analysed to screen aberrantly
expressed genes using cDNA microarray. Selected genes of those
were chosen for further analyses by immuno-histochemistry. For
this purpose a tissue array material of 114 colorectal cancer
patients was obtained. In addition to a routinely used
proliferation marker Ki-67, the analysed proteins included
securin and CDC25B.

Results: Processes such as cellular
defense, cell structure, motility and cell division were found to
be notably represented among the most deregulated genes. A
significant portion of the overexpressed genes included those
functioning in the cell cycle. Immunohistochemical stainings of
securin and CDC25B showed a consistent expression pattern
with that of cDNA microarray analysis. There was no statistical
association between the studied proliferation markers and
survival. Instead, there was a significant association between the
Dukes’ class and the histological grade (p=0.04), but not
between histological grade and survival. The survival of Dukes’
B patients was significantly poorer if no regional lymph nodes
were studied compared with the Dukes’ B patients with even a
single studied lymph node (p=0.04, hazard ratio 2.7).
Conclusion: (a) Tumour stage is superior in estimating the
prognosis of patients with colonic cancer compared with the
grading of cell cycle regulators or histological grade of the cancer
and (b) the study of regional lymph nodes is essential to identify
the patients who would benefit from adjuvant chemotherapy.

BibTeX entry:

@ARTICLE{jTaTuGrHuKrAiNeHiNeSu06a,
  title = {Biochemical and Clinical Approaches in Evaluating the Prognosis of Colonic Cancer},
  author = {Talvinen, Kati and Tuikkala, Johannes and Grönroos, Juha and Huhtinen, Heikki and Kronqvist, Pauliina and Aittokallio, Tero and Nevalainen, Olli S. and Hiekkanen, Heikki and Nevalainen, Timo and Sundström, Jari},
  journal = {Anticancer Research},
  volume = {26},
  number = {6},
  year = {2006},
  keywords = {Cell cycle, colorectal cancer, lymph node, microarray, prognosis, tumour stage},
}

Belongs to TUCS Research Unit(s): Algorithmics and Computational Intelligence Group (ACI), Biomathematics Research Unit (BIOMATH)

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