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Ovarian Endometriosis Signatures Established Through Discovery and Directed Mass Spectrometry Analysis

Anni P. Vehmas, Dorota Muth-Pawlak, Kaisa Huhtinen, Taija Saloniemi-Heinonen, Kimmo Jaakkola, Teemu D. Laajala, Heidi Kaprio, Pia A. Suvitie, Tero Aittokallio, Harri Siitari, Antti Perheentupa, Matti Poutanen, Garry L. Corthals, Ovarian Endometriosis Signatures Established Through Discovery and Directed Mass Spectrometry Analysis. Journal of Proteome Research 13, 4983–4994, 2014.

Abstract:

New molecular information on potential therapeutic
targets or tools for noninvasive diagnosis for
endometriosis are important for patient care and treatment.
However, surprisingly few efforts have described endometriosis
at the protein level. In this work we enumerate the proteins in
patient endometrium and ovarian endometrioma by extensive
and comprehensive analysis of minute amounts of cryosectioned
tissues in a three-tiered mass spectrometric approach.
Quantitative comparison of the tissues revealed 214 differentially
expressed proteins in ovarian endometrioma and endometrium.
These proteins are reported here as a resource of SRM (selected
reaction monitoring) assays that are unique, standardized, and openly available. Pathway analysis of the proteome measurements
revealed a potential role for Transforming growth factor β-1 in ovarian endometriosis development. Subsequent mRNA
microarray analysis further revealed clear ovarian endometrioma specificity for a subset of these proteins, which was also
supported by further in silico studies. In this process two important proteins emerged, Calponin-1 and EMILIN-1, that were
additionally confirmed in ovarian endometrioma tissues by immunohistochemistry and Western blotting. This study provides the
most comprehensive molecular description of ovarian endometriosis to date and researchers with new molecular methods and
tools for high throughput patient screening using the SRM assays.

BibTeX entry:

@ARTICLE{jVeMuHuSaJaLaKaSuAiSiPePoCo14a,
  title = {Ovarian Endometriosis Signatures Established Through Discovery and Directed Mass Spectrometry Analysis},
  author = {Vehmas, Anni P. and Muth-Pawlak, Dorota and Huhtinen, Kaisa and Saloniemi-Heinonen, Taija and Jaakkola, Kimmo and Laajala, Teemu D. and Kaprio, Heidi and Suvitie, Pia A. and Aittokallio, Tero and Siitari, Harri and Perheentupa, Antti and Poutanen, Matti and Corthals, Garry L.},
  journal = {Journal of Proteome Research},
  volume = {13},
  pages = { 4983–4994},
  year = {2014},
  keywords = {endometriosis, tissue, AIMS, proteotypic peptides, mass spectrometry, quantitative proteomics, Western blot, immunohistochemistry, selected reaction monitoring, SRM},
}

Belongs to TUCS Research Unit(s): Biomathematics Research Unit (BIOMATH)

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